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Plasmid DNA Purification Kit(Recommend)
| Offer Type: |
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| Offer Post Time: |
2008-07-11 |
| Expiration Date: |
2008-09-01 |
| Details: |
Description:
The Plasmid DNA Purification Kit is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. The Kit combines the power of silica membrane technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high quality DNA in under half an hour. The silica membrane reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer. Plasmid DNA Purification Kit mini-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. Yields vary according to plasmid copy number, E. Coli strain, and conditions of growth, but 1~5 ml ml of overnight culture in LB medium typically produces 1~40 g plasmid DNA. The plasmid DNA purified by this kit can be directly used for most molecular biology experiments such as restriction enzyme digestion, PCR, in vitro transcription and DNA sequencing.
Features
1. Unique adsorption material: Bind nucleic acid DNA more efficiently and more uniquely.
2. High yield: Up to 40¦Ìg pure plasmid DNA can be purified from 1-5ml sample of bacteria medium.
3. High purity: 1.84. Fast and simple protocol: Purify plasmid DNA in 20 minutes.
5. Safety: No phenol and chloroform required. |
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