ASCEN High Quality Disposable Sperm Counting Slide For CASA Semen Analysis
ASCEN Sperm Counting Slide Main Advantage
ASCEN slides are high quality disposable counting chambers. These slides were especially developed for CASA semen analysis. Other uses have been found in many different fields like haematology and microbiology for example.
1. Non-toxic.The ASCEN slide is made using non-toxic glue, ink and coating. Samples therefore remain viable and can be counted for up to 45 minutes after loading the chamber.
2. No bubbles.Due to the consistently accurate chamber depth and specially developed coating, no air bubbles will occur when the ASCEN slide is loaded with a sperm sample.
3. Less diluting.
ASCEN slides have a specially designed air vent that allows even viscose samples to spread evenly throughout the whole chamber.
ASCEN Sperm Counting Chamber Annoucements:
1. Semen collecting samples should be combined with samples after dilution, so as not to affect observation and analysis because of sperm density.
2. Before testing, sperm samples are placed on 38 ºC thermostatic temperature platform for 3 minutes, which can get more accurate test results;
3. For frozen or more viscous semen samples, it should gently tap the coverslip after sample adding to eliminate static electricity and the surface protein adhesion, spreading sperms and avoiding sperm aggregation to affect the observation and analysis;
4. The counting slides are disposable supplies. Don't reuse it and then it can protect the test results from the contamination.
CASA sperm counting slides are designed for the computer assisted sperm analysis to meet the requirements of CASA and OIE international standards.
Our ASCEN CASA disposable counting chamber slide is perfectly match with the world popular CASA Software system for automatic semen analysis.
1. Hamilton Thorne Computer Assisted Sperm Analysis CASA (Computer Assisted Sperm Analysis) image analysis systems are designed to bring quality, efficiency, and reliability to studies of reproductive cells in the human fertility*, animal sciences, and reproductive toxicology fields. Our sperm analyzers assist researchers and clinicians in analyzing sperm motility, morphology, or other characteristics in human fertility, toxicology, research, and animal applications. IVOS II CASA Sperm Analyzer, CEROS II Microscope Sperm Analyzer, TOX IVOS II CASA Semen Analyzer
2. MICROPTIC CASA Systems SCA CASA System for semen analysis allows the accurate, repetitive and automatic assessment of the following sperm parameters: motility, concentration, morphology, DNA fragmentation, vitality, acrosome reaction and leukocytes.
3. Minitube AndroVision: CASA software with PC and monitor AndroVision CASA Analyzer is a highly precise automated system for computerized semen analysis. It not only provides classical analyses of motility, concentration and morphology, but also various fluorescence based assessments of sperm functionality. The basic system for the analysis of motility and concentration is complemented by optional software modules.
4. Computer Assisted Semen Analysis System - GenePro All these CASA Comptuter Assisted Semen Analysis Software System Analyzer popular in the market
Sperm MotilitySperm concentration assessment (M/ml) and motility analysis are performed on native samples by recording AVI clips (acquired live from your imaging device to PC memory or prerecorded to disk). The analysis strictly follows the requirements of the "WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction". The analysis is based on frame by frame detection of sperm heads on video clips and building precise tracks which reveal the nature of sperm movement and provide sperm concentration value. The total number of sperms is also calculated automatically. One frame with tracks is automatically saved to database record to serve as visual support in the report or you can save a suitable frame yourself.The following parameters are calculated:- VCL = curvilinear velocity (micron/s). Time-average velocity of a sperm head along its actual curvilinear path, as perceived in two dimensions in the microscope.
- VSL = straight line velocity (micron/s). Time-average velocity of a sperm head along the straight line between its first detected position and its last.
- VAP = average path velocity (micron/s). Time-average velocity of a sperm head along its average path. This path is computed by smoothing the actual path.
- LIN = linearity. The linearity of a curvilinear path.
- STR straightness. The linearity of the average path.
- BCF = beat cross frequency (beats/s). The average rate at which the sperm's curvilinear path crosses its average path.
- ALH = amplitude of lateral head displacement. Magnitude of lateral displacement of a sperm head about its average path.
- WOB = wobble. A measure of oscillation of the actual path about the average path, VAP/VCL.
- MAD mean angular displacement of the sperm head.
- Elongation of sperm head.
Pointing with your mouse cursor to a track, you can see the exact values of the parameters for the selected spermatozoon and classification result in a pop-up window. The clip can be played again, rewound, played frame by frame for detailed analysis. You can save clips to your hard disc uncompressed without loss of quality or by using virtually any codec installed on your PC (xVid codec is recommended). You can adjust detection threshold and also change the classification limits if required.Based on the parameters mentioned above, the motility of each spermatozoon is graded A, B, C or D (WHO), according to whether it shows:- A = rapid progressive motility
- B = slow or sluggish progressive motility
- C = nonprogressive motility
- D = immotility
- A+B = progressive motility
- A+B+C = total motility
Additionally, the software enables the user to assess the concentration of:- White blood cells
- Immature germ cells
- Round cells
Flexible tool for adjustment of sperm head detection will let you work with both bright field and phase contrast (negative phase contrast yields the best results) and not only human sperms but also many animal species.Sperm MorphologyMorphology of the sperm head is an important criterion for the correct diagnosis. The software is set up to analyze still images of smears stained with the Diff-Quik stain according to strict Krueger's criteria. We have selected Diff-Quik as worldwide recognized leader in rapid staining of sperm. With Diff-Quik, the head is stained pale blue in the acrosomal region and dark blue in the post-acrosomal region which is a good basis for precise image analysis. The following parameters are assessed for every spermatozoon:- Area of the head.
- FFC = form factor circle. The degree of similarity of the sperm head to a circle.
- Perimeter of the head
- Brightness.
- ELL_B = Big axis of ellipse outlining the sperm head, the length of the sperm head.
- ELL_S = Small axis of ellipse outlining the sperm head, the width of the sperm head.
- Elng = elongation of the sperm head.
- FFE = form factor ellipse. The degree of similarity of the sperm head to an ellipse.
- Acrosome = Percentage of the acrosomal region.
The software classifies spermatozoa into Norm and Head Pathology classes automatically based on head parameters. You can easily correct the results manually and also specify other anomalies (Tail Pathology, Neck Pathology). An extended morphology classifier is available which allows you to specify the following spermatozoa structure abnormalities indicating potential infertility: tapered, pyriform, round, amorphous, vacuolated, small acrosome, double head, pinhead, bent neck, asymmetrical neck, thick insertion, thin neck, short tail, bent tail, coiled tail, excess residual cytoplasm (ERC).In case you can not receive a good image of stained smear suitable for automated detection (e.g. because of incorrect sample preparation), there is a special tool which allows you to outline the cells manually.Software is able to detect both samples prepared according to WHO recommendations for CASA including centrifugation (recommended) but also can be adjusted to easier procedures which provide higher background staining. For complicated cases, there is an option of manual drawing and deleting of objects.It is now customary to record the number of morphological sperm defects divided by the number of defective spermatozoa, a measure called the teratozoospermia index (TZI). After the analysis is finished, the TZI is calculated automatically. The teratozoospermic index values should read between 1.00 (each abnormal spermatozoon has only one defect) to 3.00 (each abnormal spermatozoon has head, neck and tail defects).Sperm VitalitySperm vitality is an important test, especially for samples with less than about 40% progressively motile sperms. The percentage of live spermatozoa is assessed by identifying those with an intact cell membrane, from dye exclusion or by hypotonic swelling. The dye exclusion method is based on the principle that damaged plasma membranes, such as those found in non-vital (dead) cells, allow entry of membrane-impermeant stains. The hypo-osmotic swelling test presumes that only cells with intact membranes (live cells) will swell in hypotonic solutions. Sperm vitality should be assessed as soon as possible after liquefaction of the semen sample, preferably at 30 minutes, but in any case, within 1 hour of ejaculation, to prevent observation of deleterious effects of dehydration or of changes in temperature on vitality. It is clinically important to know whether immotile spermatozoa are alive or dead. Vitality results should be assessed in conjunction with motility results from the same semen sample. The presence of a large proportion of vital but immotile cells may be indicative of structural defects in the flagellum. A high percentage of immotile and non-viable cells (necrozoospermia) may indicate epididymal pathology.Download Sample ReportDNA Fragmentation
Sperm DNA fragmentation test provides additional information on semen fertility potential that can not be achieved by means of standard motility, morphology and vitality tests. We have selected the Sperm Chromatin Dispersion (SCD) method as simple, fast, accurate, and highly reproducible method for the analysis of sperm DNA fragmentation in semen and processed sperm. The SCD test utilizes the idea that after acid denaturation and removal of nuclear proteins sperm with non-fragmented DNA produce the well seen halo of dispersed DNA loops while spermatozoa with fragmented DNA fail to produce such halo. When somatic cells or spermatozoa with nonfragmented DNA are immersed in an agarose matrix and directly exposed to lysing solutions, the resulting deproteinized nuclei show extended halos of DNA dispersion. The halos correspond to relaxed DNA loops attached to the residual nuclear structure. These deproteinized nuclei are called "nucleoids". The presence of DNA breaks promotes the expansion of the halo of the nucleoid and is the basis for the halo test to detect DNA damage when sperm are treated with an acid solution prior to lysis buffer, the DNA dispersion halos that are observed in sperm nuclei with nonfragmented DNA after the removal of nuclear proteins are either minimally present or not produced at all in sperm nuclei with fragmented DNA. Human Sperm Counting Chamber Application
The sperm counting chambers are apply to the computer and microscope assisted tests, such as, clinical medical examinations, animal reproductive, life science researches, drug trials, toxicology experiments for different types sperm analysis.
Usage: Using the micro pipette or pipette, it can add moderate concentration sample solution to the application area of the slide, and then the slide can automatically suck the sample into the chamber after the sample touch the edge of the cover slip.
Human Sperm Counting Slide For Semen Analysis Same Function With Leja Slide
Sperm Counting Slide
Performance:
1. Clear sperm imaging, accurate and reliable test results: According to the sizes of different sperm species, the slides can use the optimized counting chamber forming the single - layer, independent sperm cell, without overlapping and stacking phenomena. And then sperms will form a clear image on the same focal plane without affecting the freedom movement of sperm in the horiziontal direction.
2. Established the standardized test of sperm to improve work efficiency: Using the optimized exhausting design, the counting chamber can automatically suck the sperm from the suction port and filled the entire chamber. It can save your time and reduce the working intensity.
3. Large amount of detection but low cost: each sperm counting slide is designed with 2 to 8 seperate testing chambers. Each counting slide may simultaneously test 2 to 8 specimens which can help you save your money. Ordinarily, four tests on one slide.
4. One time only, no need to wash for reuse. It can prevent cross pollution from repeated use.
5. Fast heating up to the set temperature to protect the motility and vitality of spermwith high detection efficiency and more accurate results.
6. It designed for the computer assisted sperm analysis to meet the requirements of CASA and OIE international standards.
7. Applications: It suitable for the clinical medical examinations, animal husbandries, life science researches, drug trials, toxicology experiments and many analysis of different types sperm.
Detailed Images
Instructions
1. The body fluid placement table is located at Φ8mm on the base plate. After using medical alcohol to clean and disinfect, you can use it.
2. Drop the liquid to be tested on the circular carrier and close the ring cover. Place the microscope platform to observe the count.
3. In the microscope eyepiece system access digital video camera and computer connection, you can use the analysis software for counting or morphology analysis.
Technical Parameters:
1. Dimensions:74 * 36 *7mm (plate with the total thickness).
2. The gap between the carrier platform (Φ8mm) and the cover plate is 10μ (0.01mm), accuracy error, ±1.5μ at a constant temperature of 37°C.
3. Grid size: 0.1 x 0.1 = 0.01 mm per cell.
Q1. What is your terms of packing?
A: Generally, we pack our goods in neutral white boxes and brown cartons. If you have legally registered patent,
we can pack the goods in your branded boxes after getting your authorization letters.
Q2. What is your terms of payment?
A: T/T 30% as deposit, and 70% before delivery. We'll show you the photos of the products and packages
before you pay the balance.
Q3. What is your terms of delivery?
A: EXW, FOB, CFR, CIF, DDU.
Q4. How about your delivery time?
A: Generally, it will take 3 to 7 days after receiving your advance payment. The specific delivery time depends
on the items and the quantity of your order.
Q5. Do you test all your goods before delivery?
A: Yes, we have 100% test before delivery
Q6: How do you make our business long-term and good relationship?
A: 1. We keep good quality and competitive price to ensure our customers benefit;
2. We respect every customer as our friend and we sincerely do business and make friends with them,
no matter where they come from.
Q7. What is your sample policy?
A: We can supply the sample if we have ready parts in stock including shipping cost.
Product Parameters
| Specimen Types | Model | Depth of Chamber | Counting Chamber Volume(ml) | Analyzing Area/PCS |
Human Semen
Analysis Slides | SA10-1 | 10 | 3.2 | 1 |
| SA10-2 | 10 | 4.0 | 2 |
| SA10-4 | 10 | 2.2 | 4 |
| SA10-4 | 12 | 2.5 | 4 |
| SA10-8 | 10 | 1.3 | 8 |
Animal Semen
Analysis Slides
(Pig, Bull, Sheep) | SA20-2 | 20 | 7.8 | 2 |
| SA20-4 | 20 | 4.5 | 4 |
| SA20-8 | 20 | 2.4 | 8 |
Rat Semen
Analysis Slides | SA100-2 | | 40 | 2 |
| SA100-4 | | 25 | 4 |
Mouse Semen
Analysis Slides | SA100-2 | | 20 | 2 |
| SA100-4 | | 14 | 4 |
Poultry Semen
Analysis Slides | SA100-2 | | 18 | 2 |
| SA100-4 | | 12 | 4 |
Fish Sperm
Analysis Slides | SA100-2 | | 2.6 | 2 |
| SA100-4 | | 1.8 | 4 |
| AIYX Sperm Counting Chamber | Similar Sperm Counting Chamber |
| According to the different species of sperms, we design many different counting chamber to fit with it. No matter how high the sperms it is, it will be a single-deck in our chamber. | Detecting different sperms all need to test and cannot solve the problem of accumulate sperms. |
| Ease of use, do not need experience. | Need to debug and only for professional. |
| Disposable and cleaning. | Recycling and hard to keep slides clean. |
| One slide can support four sample, save time and money. | One slide just supports one sample and needs to wash by manual work. |
| Do not need to dilute, adding sperms immediately. | Do not need to dilute, adding sperms need to debug. |
| The strictly separate sealing design can prevent the cross infection. | One time one sample, recycle to clean. |
| Transparency with clean background, and sperm images are clear to count. | It would be hard to recognize under the accumulation and unclear background. |
| Mailang counting chamber is single-deck with no gridding. It designs for the detection of sperm motility. Sperms can move freely. | The thick slide can move freely, so that you cannot control the chamber very well. |
| Short time detection with high efficiency. It has fine heat-conducting property and can up to the setting temperature fastly to protect the motility of sperms. | Heating thick slide needs a long time, and be warm slowly. So that it will has bad effect on the motility of sperms. |
){kind=link}